Coding

Part:BBa_K3450007:Design

Designed by: Priya Sharma Shashaank G. Prerita Chawla   Group: iGEM20_IISER_Bhopal   (2020-10-23)


β-intimin Neae: Intimin is an important virulence factor of EHEC and mediates intimate adhesion


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 529
    Illegal PstI site found at 607
    Illegal PstI site found at 619
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 529
    Illegal PstI site found at 607
    Illegal PstI site found at 619
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 529
    Illegal PstI site found at 607
    Illegal PstI site found at 619
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 529
    Illegal PstI site found at 607
    Illegal PstI site found at 619
    Illegal PstI site found at 649
    Illegal PstI site found at 1096
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1542
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for Escherichia coli


Source

Enterohemorrhagic E. coli (EHEC) genome

References